negative atcc 51625 atcc 27840 atcc 43809 atcc 29970 atcc 15305 streptococcus pneumoniae other streptococcus spp  (ATCC)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Moraxella catarrhalis Outer Membrane Vesicles Carry ?-Lactamase and Promote Survival of Streptococcus pneumoniae and Haemophilus influenzae by Inactivating Amoxicillin ^▿

doi: 10.1128/AAC.01772-10

Figure Lengend Snippet: Clinical isolates, reference strains, and presence of bro-1 or bro-2 genes encoding M. catarrhalis β-lactamase

Article Snippet: Escherichia coli strains DH5α and BL21 were cultured in Luria-Bertani (LB) broth at 37°C in a humid atmosphere containing 5% CO 2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Clinical isolate/strain Site of isolation Age Gender Clinical manifestation MIC (μg/ml) a Amoxicillin susceptibility β-Lactamase status bro genotype Reference or source M. catarrhalis Bc5 Nasopharynx (reference strain) 0.032 Sensitive Negative 20 RH4 Blood (reference strain) 2.0 Resistant Positive bro-1 6 KR395 Tympanic cavity 66 yr Female Cough 0.064 Sensitive Negative 20 KR492 Nasopharynx 9 mo Male Otitis media 0.50 Resistant Positive bro-1 This study KR493 Nasopharynx 4 yr Female Recurring fever 16.0 Resistant Positive bro-1 This study KR522 Nasopharynx 35 yr Male Unknown 8.0 Resistant Positive bro-1 This study KR523 Nasopharynx 79 yr Male Unknown 8.0 Resistant Positive bro-2 This study KR526 Nasopharynx 33 yr Male Cough, sore throat 1.0 Resistant Positive bro-1 This study KR542 Nasopharynx 2 mo Male Unknown 6.0 Resistant Positive bro-1 This study KR923 Nasopharynx 4 yr Male Otitis media 3.0 Resistant Positive bro-1 This study H. influenzae NTHi 722 Nasopharynx (reference strain) 0.19 Sensitive Negative 34 Hib Eagan Reference strain 0.50 Sensitive Negative 10 S. pneumoniae ATCC 6303 Reference strain 0.094 Sensitive Negative 29 Open in a separate window a MICs were determined by Etest.

Techniques: Isolation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Moraxella catarrhalis Outer Membrane Vesicles Carry ?-Lactamase and Promote Survival of Streptococcus pneumoniae and Haemophilus influenzae by Inactivating Amoxicillin ^▿

doi: 10.1128/AAC.01772-10

Figure Lengend Snippet: M. catarrhalis β-lactamase-containing OMVs protect S. pneumoniae (pneumococci [Pnc]) from being killed by amoxicillin. Amoxicillin-susceptible S. pneumoniae ATCC 6303 (106 CFU/ml) was grown with amoxicillin (1 μg/ml) preincubated with either 25 μg/ml β-lactamase-positive OMVs (A and B) or β-lactamase-negative OMVs (B and C). OMVs were isolated from M. catarrhalis KR526 (β-lac+) and Bc5 (β-lac−). Growth was expressed either as relative growth compared to starting concentrations measured as absorbance (OD600) (A and C) or as numbers of CFU (B). The data are presented as means and the standard errors of at least three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.

Article Snippet: Escherichia coli strains DH5α and BL21 were cultured in Luria-Bertani (LB) broth at 37°C in a humid atmosphere containing 5% CO 2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Clinical isolate/strain Site of isolation Age Gender Clinical manifestation MIC (μg/ml) a Amoxicillin susceptibility β-Lactamase status bro genotype Reference or source M. catarrhalis Bc5 Nasopharynx (reference strain) 0.032 Sensitive Negative 20 RH4 Blood (reference strain) 2.0 Resistant Positive bro-1 6 KR395 Tympanic cavity 66 yr Female Cough 0.064 Sensitive Negative 20 KR492 Nasopharynx 9 mo Male Otitis media 0.50 Resistant Positive bro-1 This study KR493 Nasopharynx 4 yr Female Recurring fever 16.0 Resistant Positive bro-1 This study KR522 Nasopharynx 35 yr Male Unknown 8.0 Resistant Positive bro-1 This study KR523 Nasopharynx 79 yr Male Unknown 8.0 Resistant Positive bro-2 This study KR526 Nasopharynx 33 yr Male Cough, sore throat 1.0 Resistant Positive bro-1 This study KR542 Nasopharynx 2 mo Male Unknown 6.0 Resistant Positive bro-1 This study KR923 Nasopharynx 4 yr Male Otitis media 3.0 Resistant Positive bro-1 This study H. influenzae NTHi 722 Nasopharynx (reference strain) 0.19 Sensitive Negative 34 Hib Eagan Reference strain 0.50 Sensitive Negative 10 S. pneumoniae ATCC 6303 Reference strain 0.094 Sensitive Negative 29 Open in a separate window a MICs were determined by Etest.

Techniques: Isolation